Coding
Hag-SpyT

Part:BBa_K4202006:Design

Designed by: ZIHANG YE   Group: iGEM22_ZJU-China   (2022-09-25)


This part is a fusion protein of Hag and SpyTag , and it`s used for the bio-scafford.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 568


Design Notes

The proper site should be chosen for the insertion of SpyTag.


Source

We obtain the sequence of Hag from the <a herf="http://subtiwiki.uni-goettingen.de/">subtiwiki</a> and design the fusion protein based on the former research.The plasmid was synthesized by GenScript.

References